oasis hlb 1cc Search Results


h5000 hc heating block oasis hlb 1 cc vac cartridge  (AutoMate Scientific Inc)
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AutoMate Scientific Inc h5000 hc heating block oasis hlb 1 cc vac cartridge
Table of Materials
H5000 Hc Heating Block Oasis Hlb 1 Cc Vac Cartridge, supplied by AutoMate Scientific Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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strong-cation exchange (scx) spintips  (Protea Bio)
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Protea Bio strong-cation exchange (scx) spintips
Table of Materials
Strong Cation Exchange (Scx) Spintips, supplied by Protea Bio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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amicon ® ultra-0.5  (Merck KGaA)
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Merck KGaA amicon ® ultra-0.5
Table of Materials
Amicon ® Ultra 0.5, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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titansphere (10μm)  (GL Sciences)
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GL Sciences titansphere (10μm)
Table of Materials
Titansphere (10μm), supplied by GL Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sequence grade trypsin  (Promega)
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Promega sequence grade trypsin
Table of Materials
Sequence Grade Trypsin, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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formaldehyded2  (Cambridge Isotope Laboratories)
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Cambridge Isotope Laboratories formaldehyded2
Table of Materials
Formaldehyded2, supplied by Cambridge Isotope Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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lichrolut sample preparation unit with drying attachment  (EM Science Inc)
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EM Science Inc lichrolut sample preparation unit with drying attachment
Table of Materials
Lichrolut Sample Preparation Unit With Drying Attachment, supplied by EM Science Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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trypsin promega v511a  (Promega)
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Promega trypsin promega v511a
Table of Materials
Trypsin Promega V511a, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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lichrosep, 1ml cartridges  (Merck KGaA)
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Merck KGaA lichrosep, 1ml cartridges
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eppendorf refrigerated centrifuge  (Eppendorf AG)
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Eppendorf AG eppendorf refrigerated centrifuge
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Eppendorf Refrigerated Centrifuge, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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isolute® columns  (BIOTAGE)
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BIOTAGE isolute® columns
Table of Materials
Isolute® Columns, supplied by BIOTAGE, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sterile vials  (Hollister-Stier)
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Hollister-Stier sterile vials
Table of Materials
Sterile Vials, supplied by Hollister-Stier, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Table of Materials

Journal: Journal of visualized experiments : JoVE

Article Title: A Spin-Tip Enrichment Strategy for Simultaneous Analysis of N-Glycopeptides and Phosphopeptides from Human Pancreatic Tissues

doi: 10.3791/63735

Figure Lengend Snippet: Table of Materials

Article Snippet: % in H 2 O) Sigma-Aldrich 339261-100ML Used for opening emitter of pulled capillary column Iodoacetamide, BioUltra Sigma I1149-5G Protein reducing reagent MaxQuant software n/a n/a Free software used for phosphoproteomic analysis ( https://www.maxquant.org/ ) Multi-therm Shaker with heating and cooling Benchmark Scientific H5000-HC Heating block Oasis HLB 1 cc Vac Cartridge, 10 mg Sorbent per Cartridge, 30 μm, 100/pk Waters 186000383 Larger-scale cartridge desalting for tryptic digests (loading capacity approximately up to 1 mg each) OMIX C18 pipette tips, 100 μL tip, 10 – 100 μL elution volume, 1 × 96 tips Agilent A57003100 Smaller-scale packed pipette tip for desalting for enrichment elutions P-2000 Micropipette Puller Sutter Instrument Co. P-2000/F For pulling nano-capillary columns for LC-MS PhosSTOP phosphatase inhibitor tablets Sigma 4906845001 Pierce BCA Protein Assay Kit Thermo Fisher Scientific 23225 Pierce Quantitative Colorimetric Peptide Assay Thermo Fisher Scientific 23275 PolySAX LP (12 μm, pore size 300 Å) PolyLC BMSX1203 Material for strong anion-exchange chromatography used for ERLIC/conventional glycopeptide enrichment Potassium Phosphate Monobasic (Crystalline/Certified ACS) Fisher Scientific P285-500 Pressure injection cell with integrated magnetic stirplate Next Advance PC77-MAG For packing nano-capillary columns with stationary phase up to 2500 psi limit Proteome Discoverer software Thermo Fisher Scientific n/a Commercial software for proteomics anaysis (with integrated database searching software nodes) and data visualization ( https://www.thermofisher.com/us/en/home/industrial/mass-spectrometry/liquid-chromatography-mass-spectrometry-lc-ms/lc-ms-software/multi-omics-data-analysis/proteome-discoverer-software.html ) SpeedVac SC110 Vacuum Concentrator Model SC110-120 Savant n/a Centrifugal vacuum concentrator for drying samples (under heat) SDS Solution, 10% Sodium Dodecyl Sulfate Solution, Molecular Biology/Electrophoresis Fisher Scientific BP2436200 Sequencing Grade Modified Trypsin Promega V5111 Sodium Chloride (Crystalline/Certified ACS) Fisher Scientific S271-500 TopTip, Empty, 10–200 μL, Pack of 96 Glygen Corporation TT2EMT.96 Empty pipette tip with micron-sized hole used that can be used to pack chromatographic materials for enrichments, bundled with tube adapters Triethylammonium bicarbonate buffer (TEAB, 1 M, pH 8.5 (volatile)) Sigma 90360-100ML Trifluoroacetic acid, Reagent Grade, 99% Fisher Scientific 60-017-61 Tris Base (White Crystals or Crystalline Powder/Molecular Biology) Fisher Scientific BP152-500 Trypsin/Lys-C Mix, Mass Spec Grade Promega V5071 Urea (Certified ACS) Fisher Scientific U15-500 Water, Optima LC/MS Grade Fisher Scientific W64 Open in a separate window 3.2.

Techniques: Software, Centrifugation, Protease Inhibitor, Lysis, Blocking Assay, Transferring, Bicinchoninic Acid Protein Assay, Chromatography, Injection, Sequencing, Modification, Mass Spectrometry